HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

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HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

HG Car Dashboard Cleaner, For a Shiny Interior, Cleans & Restores Shine, Leather & Plastic Safe, Intensifies the Colour, Protects from Dust, Fresh Fragrance Polish – 400ml Spray (536040106)

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According to a study performed by Fuchs et al. in 2021, it has been found that, especially in women, the risk has been found to start at moderate alcohol consumption, and alcohol withdrawal has been found to promote the reduction in BP in short-term trials [ 52].Suliga et al., in their cross-sectional study of 12,285 individuals in the age group 37 to 66, described that daily alcohol consumption ranging from 0.1 g to 15 g was inversely related to the development of HTN in women, with an odds ratio of 0.67, 95% confidence interval of 0.59 to 0.75, p-value <0.001 [ 53]. However, in men, this relation was not observed [ 53]. Enter [email protected] as the Account name and then enter or paste the manual entry code in from above as the 'Secret key'.

The modification in functional groups present in the culture pellets was identified by measuring the spectra in the range of 400 to 4000 cm -1 using Fourier transform infrared (FT-IR) spectroscopy (IR Affinity-I spectrometer, Shimadzu, Japan), as described by Joshi etal. (2021). In brief, 48 h grown cultures in ZMB medium without Hg supplementation were used as a control, whereas cultures with 50 mg/L of Hg supplementation were used as Hg treated. The mixtures (lyophilized cells and 2% KBr) were fixed in the FT-IR spectrometer after compressing them into translucent sample discs, followed by analyzing in ATR-FT-IR mode by following the manufacturer’s protocol. Scanning Electron Microscopy (SEM) analysis of MRB When the isolate NIOT-EQR_J7 was grown in the presence of Hg 2+ supplementation, a slight reduction in the peak intensity attributed to the thiol group (S-H stretch) at 2383.09 cm -1 was observed as compared to the control along with the wavelength that shifted to 2442.42 cm -1. In contrast, the peak intensity corresponding to the thiol group of NIOT-EQR_J248 at 2304.55 cm -1 increased compared to without Hg 2+ supplementation and the shift in wavelength to 2246.72 cm -1 was observed. In the case of NIOT-EQR_J251, the peaks corresponding to the S-H group appeared at 2341.34 cm -1 in the presence of Hg 2+ whereas, in the absence of Hg 2+, the peak was negligible. The peak was absent in both the presence and absence of Hg 2+ in the case of isolate NIOT-EQR_J258 ( Figures5A–D). In accord with our findings, previous studies had also reported similar results ( Dash and Das, 2014; De etal., 2014; Joshi etal., 2021) and suggested significant changes in the FT-IR spectrum such as a shift in wavenumber in the region of O-H groups, –S-H group, amide C=O, Nitrile C–N stretch, phosphate groups, etc. The changes in peak height and area have also been observed when isolates were grown in the presence of Hg 2+ ions compared to the controlled ones. The transmittance of the infrared (IR) in the presence of Hg 2+ was lower than in the absence of Hg 2+. The changes suggested that the presence of metal leads to a lesser degree of bond stretching, consequently reducing the IR transmittance. The contribution of functional groups in metal binding was legitimized undeniably by the formation of varying spectral bands with and without Hg ( Oves etal., 2013). Hg volatilization by bacteria involves the reduction of Hg 2+ via the mer system to Hg 0, which is less toxic. It is appealing from the essential and practical viewpoints that halophilic bacteria can also grow in the presence of a higher amount of Hg and volatilized Hg efficiently similar to the non-halophilic bacteria. The ICP-MS analysis did not detect significant Hg loss in the control sample (without bacteria), which confirms the volatility of Hg being biotic ( Table S2). Thus, our results suggested that halophilic bacteria can also remove the Hg from the growth media, indicating that it may be potentially applied in contaminated environments. The Hg comes into the ocean either through atmospheric deposition or other several natural and active or passive processes. The results of this study imply that the two marine strains of Halomonas sp., isolated from ERIO may have the ability to remove Hg 2+ from the growth medium. Identification of functional groups changed during Hg(II) reduction The health effects of alcohol intake are variable and are based on the amount of intake (low, moderate, or heavy) and intake pattern (acute, chronic, or binge) [ 45- 47]. In the US, the amount of pure alcohol in one standard drink is 14 g, which can be found in 12 oz of regular beer (about 5% alcohol), 5 oz of wine (about 12% alcohol), and 1.5 oz of distilled spirits (about 40% alcohol) [ 48]. Moderate alcohol intake recommendations according to the Dietary Guidelines for Americans are the consumption of two standard drinks per day for men and one standard drink per day for women [ 49]. Moderate alcoholic intake has been shown to reduce the risk of chronic disease [ 49]. Three experimental replicates were determined for each analysis and the statistical significance of the results was analyzed by one way ANOVA test. All Statistical analyses were carried out using SPSS (version 17) and GraphPad Prism 7 software. Differences were considered significant at p< 0.05 and values are reported as mean ± standard deviation (SD). Results and discussion

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Assorted studies have shown that IF lowers BP. Harvie et al., in their study involving 107 overweight or obese premenopausal women, showed that IF for six months helped lower SBP (p= 0.99) and DBP (p= 0.84) [ 22]. Varady et al., in their study performed for 12 weeks with IF involving 15 overweight individuals (five males, 10 females with a BMI of 20-29.9 kg/m 2 showed that IF helped lower BP with a p-value of 0.51 [ 23]. A study by Bhutani et al. involving 83 obese individuals (three males and 80 females) with a BMI of 30-39.9 kg/m 2revealed that 12 weeks of IF helped lower SBP (p = 0.254) and DBP (p = 0.570) [ 24]. In a study by Eshghinia et al., 15 overweight or obese women with a BMI ≥25 kg/m 2 who followed IF for eight weeks showed a lowering of SBP (p<0.001) and DBP (p<0.05) [ 25]. Teng et al., in their 12 weeks IF study with 28 Malay men with a BMI of 23-29.9 kg/m 2,showed a lowering in SBP (p<0.05) and DBP (p<0.05) [ 26]. Erdem et al., in their study involving 60 participants from the Cappadocia cohort with pre-HTN and HTN with SBP of 120-139 and more than or equal to 140 mm Hg, DBP of 80-80 and more than or equal to 90 mm Hg, revealed that IF helps lower SBP (p<0.001) and DBP (p<0.039) [ 27]. Mercury (Hg) pollution poses a global threat to human and environmental health due to its noxiousness, mobility, and lengthy residence duration in the atmosphere ( Raphael etal., 2011). Toxic metals rapidly accumulate in the food chain, impacting higher trophic levels, hence these are of principal concern nowadays ( Raphael etal., 2011). According to recent research, oceanic release and biomass burning (organic compounds) account for the majority of worldwide Hg emissions, with anthropogenic activities accounting for the remaining significant percentage ( Pirrone etal., 2010; Nelson etal., 2012; Serrano etal., 2013). Hg exists in elemental, inorganic, and organic forms in both land and water systems, depending on oxidation-reduction conditions. In the atmosphere, Hg with valence +2 is more extensively spread ( Wang etal., 2004). In the biogeochemical cycle, a considerable part of Hg accumulates in seas and oceans by its atmospheric deposition ( Bindler, 2003; Wang etal., 2004). The effects of human-induced sources on the Hg contents and its forms are extensively larger in the photic layers of oceans ( Strode etal., 2007).

The pathogenesis of HTN involves oxidative stress. Another mechanism involved is the decreased bioavailability of nitric oxide (NO) [ 38]. Physical exercise could be a potential lifestyle intervention to treat HTN due to its beneficial effects on endothelial function and oxidative stress [ 38]. Exercise exerts an anti-inflammatory action via the hypothalamic-pituitary-adrenal axis and via the sympathetic nervous system, thus affecting BP directly [ 9]. The physiologic effects of exercise are further divided into acute, post-exercise, and chronic [ 9]. Aerobic exercises like speed walking, jogging, running, cycling, dancing, and swimming have been shown to decrease resting BP and BP reactivity to stressors [ 9]. A study by Ozemek et al. revealed the following about how diverse types of exercises affect BP: (1) Aerobic exercise of 90 to 150 minutes per week with 65%-75% heart rate reserve has been shown to impact SBP by −5/8 mm Hg in hypertensive individuals and by −2/4 mm Hg in normotensive individuals. (2) Dynamic resistance exercise of 90 to 150 minutes per week with 50%-80% one rep maximum, six exercises, three sets/exercise, and ten repetitions/set has been shown to decrease SBP by 4 mm Hg in hypertensive individuals and 2 mm Hg in normotensive individuals. (3) Isometric resistance exercise of 4 × 2 min (hand grip), 1 min rest between exercises, 30%-40% maximum voluntary contraction, and three sessions per week for 8-10 weeks have been shown to lower SBP by 5 mm Hg in hypertensive individuals and 4 mm Hg in normotensive individuals [ 11].

Data availability statement

Based on the results obtained by the x-ray film method, ICP-MS analysis has been carried out to confirm the Hg removal potential of the isolates in the presence of 50 mg/L of Hg 2+. The ICP-MS outcomes showed significant changes in the level of Hg depletion. Among all, the marine culture NIOT-EQR_J251 possessed the highest potential (70.62%) to volatilize Hg. NIOT-EQR_J258 showed the least removal of Hg (17.48%), whereas NIOT-EQR_J7 and NIOT-EQR_J248 were capable to volatilize 29.18% and 52.17% of Hg 2+ under similar conditions, respectively. ( Figure4B). NIOT-EQR_J251 could volatilize 32.54 mg of the initial inoculum of 46.07 mg of HgCl 2, whereas NIOT-EQR_J258 removes 8.03 mg of the initial supplement of 45.74 mg of Hg 2+. In presence of 50 mg/L of Hg 2+, NIOT-EQR_J7 and NIOT-EQR_J248 dominantly developed the fog on the x-ray film, whereas, in the ICP-MS analysis, NIOT-EQR_J251 showed the highest Hg removal as compared to the other isolates. This suggests that volatilization was not solely responsible for removing the Hg 2+ from the culture media and the contribution of different processes like bioaccumulation and bio-adsorption may be involved.

The Mediterranean diet (Med Diet) has also helped lower BP. As per the Maine-Syracuse Longitudinal Study conducted in the United States in 2020 by a group of researchers who followed 851 US older adults, for every one unit increase in the Med Diet score in participants, it was found that there was a corresponding reduction of 0.69 units in SBP, a reduction of 0.33 in DBP, and a reduction of 0.45 on mean arterial pressure (MAP) [ 32]. Although this seems to be small, this change can have a noteworthy effect at the level of the population; that is, a decrease of 2 mm Hg in SBP can lead to a decrease of 10% when it comes to the population [ 32]. According to the observational studies conducted in Mediterranean countries, higher adherence to a Med Diet is associated with a decreased risk of cardiovascular disease, overall mortality as well as neoplastic disease [ 33, 34]. The Med Diet consists of higher consumption of extra virgin olive oil, vegetables, fruits, whole grains, nuts, cereals, as well as seeds; moderate consumption of fish, poultry, red wine, and dairy; and lower consumption of processed foods and red meat [ 35]. In prior studies, many pathways have been reported for Hg 2+ detoxification in microbes using various tools and technologies ( Chang etal., 2020; Cursino etal., 2000; Chang etal., 2021). In the present study, a metabolic pathway is hypothesized based on the GC-MS metabolites and protein-ligand interaction ( Figure S6) and an attempt has been made to correlate the proposed pathway with the different components of the already existing pathways. In this preliminary study, we found that general resistance/detoxification mechanisms of NIOT-EQR_J248 and NIOT-EQR_J251 in response to inorganic mercury (Hg 2+) exposure were a multisystem combined process. The statistical analysis of Hg removal potential among the different species i.e., Alcanivorax xenomutans, Halomonas sp., Marinobacter hydrocarbonoclasticus and within the Halomonas spp. revealed that the Hg removal by NIOT-EQR_J251 was significantly higher (p< 0.01) than NIOT-EQR_J7 and NIOT-EQR_J258. There was a significant difference (p< 0.05) in the removal of Hg between NIOT-EQR_J248 vs. NIOT-EQR_J258 and NIOT-EQR_J248 vs. NIOT-EQR_J251. Al-Mailem etal. (2011) reported Haloferax sp. (HA1 and HA2), Halobacterium sp. HA3, and Halococcus sp. HA4 effectively volatilized (from 40 to 65%) the available 100 mg/L of Hg after 8 days. Many other isolates such as Bacillus sp., Pseudomonas stutzeri, Pseudomonas putida, Vibrio fluvialis could volatilize 60%-95%, 94%, 100%, 60% of Hg 2+, respectively, from culture ( Zhang etal., 2012; Dash etal., 2013; Giri etal., 2014; Saranya etal., 2017; Zheng etal., 2018).The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material. Author contributions HG car dashboard cleaner makes the interior of the car look like new. This dash cleaner cleans and protects synthetic materials, plastic, rubber, vinyl and leather. Also, the car dash cleaner intensifies the colour and restores the shine of the polished surface. The HG dashboard spray also leaves a wonderfully fresh fragrance after the treatment, but not a detectable greasy coating. How do you use HG car dashboard cleaner? GC-MS analysis was performed to characterize the bacterial response and the metabolomic changes leading to Hg tolerance. The solvent extraction method was used to extract the bacterial metabolites. In brief, the freshly inoculated and exponentially grown MRB and MMRB bacterial cells in ZMB medium (non-exposed and exposed to 50 mg/L of Hg) were freeze-dried using BENCHTOP lyophilizer (VIRTIS Instrument, Gardiner, NY). For extraction of compounds, 50 mg of lyophilized bacterial cells were suspended in ethyl acetate and chloroform (1:1; v/v) and homogenized. After homogenization, the solution (crude extract) containing the metabolites was transferred to the clean glass vial by pipetting. These steps were repeated two-three times to obtain a pure and ample amount of sample. The separated organic fractions (crude extract) were treated with anhydrous NaSO 4 (Sigma-Aldrich) to remove moisture, which was again concentrated on the rotary evaporator (BUCHI Rotavapor R-215/V advanced, Switzerland) at RT and stored at -80°C until further analysis. The concentrated crude extract was re-suspended in 1 mL of Dichloromethane (DCM) and 5 µL of the sample was injected into the GC-MS analyzer (Agilent Technologies Instrument 7890A GC System, 240 Ion Trap GC/MS, USA). The GC-MS analysis was carried out under external ionization mode using a fused silica column HP 5 MS column (30 m × 0.320 mm × 0.25 µm). High purity helium was used as a carrier gas at a constant flow rate of 1 mL/min. For analysis, the chromatographic conditions i.e. initial injector and detector temperature, were set at 250°C and 330°C, respectively. The temperature of the column was programmed from 50°C (hold for 2 min) to 320°C (2 min hold), with a constant 5°C increment per minute and 1 min hold at 330°C. A metabolic library of all the separated compounds found via GC-MS analysis of bacterial extract was created and identified using NIST mass spectral library match. The PubChem CID, structures, names, and molecular weight of those bioactive compounds were obtained from the PubChem database. In-silico analysis

The following four randomized controlled trials have been conducted to show that exercise helped lower both systolic and diastolic BP in participants: In the meta-analyses of randomized controlled trials conducted by Fagard et al., 72 trials have been conducted with an average of 40 participants per trial, which involved a 16-week study of 40 minutes of exercise sessions three times/week with an average intensity of 65% of heart rate [ 39]. This study showed a decrease in SBP of 6.9 mm Hg and a lowering of DBP of 4.9 mm Hg [ 39]. In 27 randomized controlled trials by Lee et al. with 1842 participants, the exercise regimen involved walking for 26.5 min/day for 4.4 days/week for a mean of 19 weeks [ 40]. This study shows that there is a larger effect with more intense and frequent exercise regimens for a longer duration [ 40]. There was a mean decrease in SBP of 5.2 to 11 mm Hg and in DBP of 3.8 to 7.7 mm Hg [ 40]. In the meta-analysis of randomized control trials performed by Cornelissen et al., 15 trials with 633 participants involving the exercise of 30-60 min, two to five times/week, at 50% to 75% HR reserve for six to 52 weeks showed a daytime decrease in SBP of 3.2 mm Hg and in DBP of 2.7 mm Hg [ 41]. However, no blood pressure reduction was seen at night [ 42]. The meta-analysis of randomized controlled trials performed by Cornelissen and Smart included 105 trials with 3957 participants [ 42]. This study concluded that moderate aerobic exercise involving walking and jogging for 30 to 60 min/session three to five times/week for four to 52 weeks showed a reduction in SBP of 3.5 mm Hg and in DBP of 2.5 mm Hg [ 42]. Briasoulis et al., in their meta-analysis of 16 prospective long-term studies, revealed that alcohol consumption of more than 20 g per day increases the risk of HTN significantly in women, whereas consumption of 31 to 40 g in men significantly increases the risk of HTN [ 54]. Nevertheless, a higher risk of HTN was observed in all individuals who consumed more than 20 g of alcohol per day, irrespective of their gender [ 54]. Ozemek et al. revealed in their study that, in persons who consume alcohol, a reduction to two standard drinks or less daily in men and one drink or less daily in women has been shown to reduce SBP by 4 mm Hg in hypertensive individuals and by 3 mm Hg in normotensive individuals [ 11].

Based on phenotype characteristics, a total of 162 bacterial colonies grown on the ZMA media supplemented with 10 mg/L of Hg as HgCl 2 were selected for further analysis. In the presence of 25 mg/L of Hg 2+, only 63 isolates out of 162 showed resistance. Further, these 63 isolates were characterized based on their growth pattern in the presence of more than 25 mg/L i.e. 50, 75, and 100 mg/L of Hg 2+ concentration. Among the 63 isolates, 21 isolates grew in the presence of 50 mg/L, followed by 9 and 4 isolates at 75 and 100 mg/L of HgCl 2, respectively. The growth pattern of 4 isolates in the presence of HgCl 2 is shown in Figure S1. Hg tolerance of CMRBs was calculated by the broth dilution method ( Konopka and Zakharova, 1999; Santos-Gandelman etal., 2014) with some modifications. CMRB cultures grown in Zobell Marine Broth medium (ZMB, Himedia, Mumbai) without Hg supplementation were used as a positive control, whereas ZMB treated with Hg (without bacteria) was used as a negative control in this study. To ensure the uniform bioavailability of Hg to the bacteria, uniform culture conditions were maintained across different experimental treatments. Based on the growth behavior of cultures in the presence of a higher concentration of HgCl 2, the selected isolates were classified into two different categories i.e. MRB and moderate MRB (MMRB). The detailed procedure is elaborated in the SI. To understand the effects of different Hg concentrations on bacterial growth, two qualitative analyses were carried out, i.e. monitoring the optical density (OD) at 600 nm and dry biomass. 16S rDNA based identification Now the top of the interior is clean, it's time to shake and beat the mats. That will deal with the larger bits of dirt. Patients can find it increasingly difficult to maintain long-term dietary changes like low caloric intake, low salt intake, and limiting processed foods, which in turn may lead to a shift towards preintervention practices in some individuals, for example, increased calorie intake [ 28]. This implies the need for interventions to focus on ways to help patients maintain healthy dietary patterns [ 28]. To inspect the consequence of Hg 2+ concentration on the morphology, the bacterial cells were freshly grown in the presence (50 and 100 mg/L - test samples) and absence (control) of Hg 2+ as HgCl 2; and cell pellets were harvested by centrifugation (5000 rpm at 4°C for 5 min) after 48 h. SEM analysis has been carried out, as reported by Joshi etal. (2021). In brief, the bacterial cells were fixed with 2.5% glutaraldehyde followed by the post-fixing in 1% osmium tetroxide and dehydration of the cells with graded ethanol series (25%, 50%, 80%, 90%, and absolute). The processed samples were scanned using SEM (JEOL-JSM-IT500). Extraction of metabolites and Gas Chromatography-Mass Spectrometry (GC-MS) analysis



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